Medicinal plants have rich traditional utilization history in India; Ayurveda, Siddha, Unani and Folk healthcare system. The present study entitled, ‘Ethnopharmacological Validation of Traditionally Used Medicinal Plants in India: Phytochemical and Antimicrobial Evaluation' has reviewed the literature about the importance of traditionally used medicinal plants in India. The main aim of the study is to elucidate traditional uses, highlight the major phytochemical constituents of selected medicinal plants and explore their reported antibacterial and antifungal activity. A secondary-data based research methodology was employed and the research articles, scientific journals, books, review papers, government publications, and a genuine online database were used as the data sources. The review emphasizes on the most significant phytochemical classes such as alkaloids, flavonoids, tannins, saponins, terpenoids and phenolic compounds that have been linked to several biological activities in the past. Traditional plants like neem, tulsi, turmeric, garlic, aloe vera and other medicinal plants are discussed in relation to their antimicrobial potential. The study also highlights the fact that the antimicrobial activity can be different based on plant species, plant parts, and methods of extraction, solvents, concentrations and microorganisms tested. Findings show that Ethnopharmacological research is a useful link between traditional knowledge and modern science. But, laboratory evidence does not prove clinical efficacy or safety. To ensure the safe development and proper therapeutic validation of plant-based medicines, further phytochemical characterization, toxicological studies, standardization, pharmacological investigations and clinical research is required.

Pankaj Singh Nishad, Dr. Goverdhan Singh Thakur, Dr. Shriram Kunjam,"Ethnopharmacological Validation of Traditionally Used Medicinal Plants in India: Phytochemical and Antimicrobial Evaluation" International Journal of Advanced Multidisciplinary Research and Educational Development, V2(5): Page(696-705) September - October 2026. ISSN: 3107-6513. www.ijamred.com. Published by Scientific and Academic Research Publishing.